The present study was designed to investigate effects of Chinese punctuations on the comprehension of ambiguous sentence caused by different Layers, and the interaction between punctuations and the context. Experiments varied the meaning of the ambiguous sentencs according to the location of the comma, and the Consistency between the context and the meaning explained by the comma varied according to the context. The result showed: the commas in Chinese sentences could promote the processing of the reading. When there was a comma, subjects would more relay the punctuations on the understanding. The interaction between the punctuations and the context took place on the late stage.