The research surveyed 600 rural migrant workers in Suzhou，and using the situation questionnaire of rural migrant workers’ perceived social support which made by ourselves and the scale of self-esteem 、the scale of subjective well-being of rural migrant workers which have been modified in order to survey the relationship among perceived social support 、self-esteem and subjective well-being. And this research also explored the mediating influence of the self-esteem on the relation between perceived social support and subjective well-being of contemporary rural migrant workers. The research results show that：(1) The subjective well-being and its each dimensions、perceived social support of the rural migrant workers’ who with higher level of the self-esteem significantly higher than whom with lower level of the self-esteem;(2) There was significant correlation among perceived social support 、self-esteem and subjective well-being.(3) There was opposite positive correlation among perceived social support 、self-esteem and subjective well-being.(4)The self-esteem based on perceived social support has significant regression influence on the subjective well-being and its two dimensions, and it’s an important intervening variable between them.