Objective: The use of biofeedback therapy on intervention for test anxiety in university students. Methods: In this study, we selected randomly a comprehensive university of 1,500 students using the Test Anxiety Inventory (Test Anxiety scale, TAS) for evaluation, according to survey results, who were divided into two groups: anxiety group ( n = 30) and control group ( n = 30).The anxiety group and control group respectively received 40 minutes biofeedback intervention 2 times per week for 4 weeks. The use of Physiological indicators including EEG relative power a wave, electromyography (EMG) and skin conductance(SC) were detected together. Results : 1. After 4 weeks of continuous treatment, anxiety group received before and after intervention α wave EEG relative power, electromyography (EMG), and skin conductance exists differences; 2. Anxiety group of students before and after treatment on the physiological significance also exist; 3. Biofeedback therapy for test anxiety in different from the sexes, girls superior to boys. Conclusion: Biofeedback therapy can effectively reduce the level of test anxiety in university students.