Nowadays, more and more elderly people get information by surfing the internet. Previous studies have shown that color preference change with ageing. However, the majority of web pages are designed according to the color preference of the young adults ignoring the color preference of the elderly, though significant difference is existed. It is true, a lot of research has investigated the difference of the abstract color preference between two groups, and they find significant sex and age-related difference on color preference, but their results are too ambiguous to be taken into account directly in web page design for the elderly, partly because the abstract color preference is not the same as the specific color preference. As it is known, color preference is a psychological factor having a very important impact on the usability and the aesthetics of the web page. Objective: This paper aimed to investigate the web page`s specific color preference of the young and the old. One hundred and ninety-eight participants took part in the research .The participants who suffered from these illnesses such as Alzheimer ,Parkinson or diabetic retinopathy were excluded from the study. Methods: The paired-comparison method was employed in the research. Procedures: This research contained two experiments. The first experiment investigated the text and theme color preference of the young and the old, this part was an abstract color preference investigation. In theme color preference test, the stimulation materials were chosen from the 48 web-safe colors included in Mspaint program. At last, each group choose 6 colors they most preferred. When experiment started, the participants were asked to make paired-comparison between 6 colors that chosen before. In the text color preference test, 4 colors (red ,blue ,black, green)were chosen as the stimulation material. Experiment 1 described the order of the theme color and the text color after 42 times pair-comparison. Basing on the results of the experiment 1 ,18 web pages(combined by 6 theme .