Some studies have revealed that there can be similar category hierarchy between stereotype representation and entity category. However, existing research to the manipulation of the stereotype activating has not distinguished from superior category, subcategory to typical exemplar. Besides, more research remains in the behavior level. But the response reflects only information processing to the comprehensive results, not the specific process. The objective of current research is to explore possible category hierarchy by manipulating different activation of sex stereotype. In this study, participants were primed with these words respectively corresponding superior category, subcategory, typical exemplar and counter-stereotypical exemplar, followed by a word which was either consistent with gender stereotypes or inconsistent. Their task was to indicate whether the words matched or did not match, according to gender stereotypes. Both response times and event-related brain potentials (ERPs) were recorded during performance of the task. Results showed that: (1) stereotype incongruent word pairs were associated with larger N400 ERP amplitudes and slower response times, relative to congruent word pairs. (2) response times of activating superior category representation was shortest than subcategory than typical exemplar. (3) the largest N400 ERP amplitudes induced by mismatch in stereotype representation from priming superior category than subcategory than typical exemplar. These results suggest that there is a hierarchical structure for stereotype representation, which is from abstract category, subcategory to the typical sample. For gender stereotype, the stereotype representation is trend to preferentially activate abstract category. Furthermore, under the condition of the semantic activating, the violation of the gender stereotype induces N400 effect. Gender stereotype representation is top-down semantic classification processing by frontal lobe.