Although Chinese two-character words (2C-words) are composed of two characters, many one-character words (2R-words) consist of two radicals, with one on the left and the other on the right. This study investigates whether skilled readers process the radicals’ positional information in a 2R-word similarly to how they process the characters’ positional information in a 2C-word. In a priming task of semantic categorization, a group of college students’ reaction times to the one-character targets (e.g., 肝 [gan1] liver) were primed similarly by the semantic primes (e.g., 肺 [fei4] lung), the transposed non-words of the semantic primes [reversed(S) primes] (e.g., ), and the transposed non-words of the targets (reversed primes) (e.g., ). Their reaction times to the two-character targets (e.g., 电话 [dian4hua4] telephone) were also similarly primed by the semantic (e.g., 手机 [shou3ji1] phone), the reversed(S) (e.g., 机手), and the reversed primes (e.g., 话电). It was concluded that, similar to the case of the characters’ relative positions in a 2C-word, the radicals’ relative positions in a 2R-word do not influence the word’s early stage of semantic processing for skilled readers of Chinese. However, further studies are needed to investigate the relationships between a 2R-word, its radicals, and its radicals’ positional information