In this study, there are three parallel experiments testing the assumption of anxiety interfering shifting function of ACT and trying to explore its applicability among athletes. These experiments used three different paradigms of More-Odd shifting task testing the shifting function in 2(state anxiety: high vs. low)×2(groups: athletes vs. college students) between group design, that show a consistent trend: for college students, the high state anxiety reduced the processing efficiency, increased subjective efforts but had little effect on the accuracy; for athletes, the high state anxiety had little effect on the processing efficiency and accuracy. Therefore we concluded that: assumption of state anxiety interfering shifting function of ACT was applicable for college students, but not applicable for athletes.