As one of the most important social norms of the human society, fairness is essential for individual subsistence and social stability. Previous studies have shown that unfairness perception decreased specific trust toward the unfair partners. However, it is still unclear whether this effect could extend to unrelated strangers. Two studies were conducted to explore whether and how unfairness perception impacts on generalized trust to unrelated strangers. The results showed that (1) participants who were unfairly treated in the previous Ultimatum Game, invested less money to strangers in the following Trust Game (Study 1). (2) participants who observed unfair behavior in the previous Dictator Game as a beholder, also invested less money to strangers in the following Trust Game (Study 2). The results indicate that unfairness perception formed from previous interaction could temporarily decrease generalized trust to unrelated others in subsequent situation. The current study reveals that maintaining social norms has a dramatic influence on generalized trust and provide scientific reference for trust building and maintenance.