The Chinese economy has grown rapidly and the urban scale has expanded greatly since reformation and openness. People pervasively feel under great pressure in the cities. Self-enhancing humor has been widely used in daily life to cope with the great pressure with the development of media and network. Previous studies have shown that self-enhancing humor was positively associated with optimism, self-efficacy, perception of control and life satisfaction. These studies only used simple correlation research method. Structural equation modeling and multiple mediation analysis are used in this study to further explore their relationship. This study proposed the hypothesis that self-enhancing humor promoted life satisfaction through two chain mediators, i. e., emotional well-being and social support. Participants were 884 college students from four universities in China, with 42.9% boy students and the average age of 20.5 (SD = 1.96). The questionnaires were Self-enhancing Dimension from Humor Style Questionnaire, Positive Emotion and Negative Emotion Dimensions from Subjective Well-being Scale, Multidimensional Scale of Perceived Social Support and Life Satisfaction Scale. The principles of confidentiality, voluntary, anonymity and the answer without the difference of right or wrong were explained in the instruction one by one so that the error could be reduced as much as possible. Controlling for the effects of a single unmeasured latent method factor was used and showed no serious common method bias. Structural Equation Modeling was built. The model fit indexes and model modification test indicated that the model was acceptable. Joint significance test and Bias-corrected Nonparametric Percentile Bootstrap test showed that the chain mediational effect was significant. There were three mediational paths in the mediational model: (1)the mediational path through emotional well-being, with the effect size of 47.3%; (2)the mediational path through social support with the effect size of 9.1%; (3)the mediational path through emotional well-being and social support, with the effect size of 7.2%. The total mediational effect size was 63.6%. The effect size through emotional well-being was the strongest among the three meditational paths. Based on the Release Theory of Humor, the Broaden-and-Build Theory of Positive Emotion and the predictors of life satisfaction in collectivistic culture, this study constructed chain mediation model that self-enhancing humor promoted life satisfaction through two chain mediators of emotional well-being and social support, which presented a better explanation for the relationship between self-enhancing humor and life satisfaction. Future study can explore the different multiple mediation model in individualistic context.