The neural dynamics of prosodic processing in spoken language comprehension has drew a lot of attention. However, little is known to what extent prosodic information constrains neuro-cognitive processes of written language processing. Exploration into this issue provides a better understanding of the role of prosodic information. The present research is designed to investigate whether and how a comma and the lexical deflection influence the reading of Chinese sentences, by using eye movements recording. Eye-tracking is one of the ideal methodologies for exploring this problem because it allows for recording the fixation of various observers on various parts of the sentence in real time with relatively high temporal and spatial resolution. Three types of eye movement measures such as first fixation duration, gaze duration, and total fixation duration were examined to determine how lexical deviation and comma placement was activated and the interactions of lexical deviation and comma placement during silent reading of Chinese text. A self-paced, line-by-line, reading paradigm was used, in which participants were asked to read sentences silently and press a key each time when they want a new one to appear. Some of the sentences followed by a question which required participant to judge whether the two sentences having the same meaning. The Chinese sentences had or not had a comma insert at the position of the ambiguous structure. The experiments were conducted by manipulating the meaning biased by commas and lexical deviation. Analysis indicators were first fixation duration, gaze duration and total fixation duration. According to the analysis of fixation time on the lookzones，it was found that: when there was different lexical deflection, the ambiguous sentences disambiguated by the comma had a different paten on first fixation duration, gaze duration and total fixation duration. The main effects of the comma and the lexical deflection were significant (p﹤0.05). The interaction between the comma and the lexical deflection duration was also significant(p﹤0.05). Further simple effect inspection found that the times were shorter for the target region with a comma than that of without a comma no matter what the lexical deflection was(p﹤0.05). The lexical deflection had an affect on sentences processing only when the comma had biased the adjective modify the entire phrase(p﹤0.05). The results showed that the lexical and comma deflection both had an impact on sentences processing at the early stage. The insertion of commas shortened the fixation times in region when the lexical deflection in accordance with the direction the comma disambiguated to. The interaction between the comma and the lexical deflection took place at the early stage of processing. The comma had an affect on sentences processing no matter what the lexical deflection was. The lexical deflection had an affect on sentences processing only when the comma had biased the adjective modify the entire phrase. In conclusion, our data were not only compatible with the implicit prosody hypothesis, but also suggested the interaction between the suprasegmental cues and the lexical deflection occurred at the early processing during sentence reading.