The study aims to explore the difference between implicit and explicit cue preference in romance choice in college students. Based on interview and questionnaire research, the first study selects fourteen cues that regarded as important in mate decision and choose 38 males and 52 females to make important ratings of these cues in order to make a comparision between different sexes of which one counts more. The second study chooses eight most important cues respectively of different genders, and makes a distinction of the important cue rating between implicit and explicit cue preferences attained by eye tracker and self-reported survey, which used 21male college students and 24 ones who has a romantic relationship sustained above six months. The first study shows there is significant differences in cues below of distinct gender: behavior, mastery, character, profession, economic level, family and education background and. The second one indicated notable rating difference in some cues of importance in different genders. What’s more, there was significant difference of cue rating in mean duration time and cue importance when females choose their heterosexual partner, while for subjects in different genders, it existed notable differences in some cues in cue rating between average pupil size and implicit cue importance. In all, the result shows that the notable difference between college students’ implicit and explicit cue preferences in romance choices when choosing heterosexual partners of different genders. Meanwhile, some implicit index such as average duration time and pupil size can be considered as important figures in illustrating implicit attitude in romantic decision.