The development of knowledge acquired in implicit learning is an important feature of implicit learning. Implicit sequence learning , one of the important paradigms of implicit learning, consists of general motor skill learning and sequence-specific learning. This study mainly focused on sequence-specific learning. The goal of this study was to investigate the development and forgetting characteristics of knowledge acquired in implicit sequence learning through sequence reaction time paradigm. Experiment 1 adopted one-factor completely random design to investigate the development of knowledge acquired in implicit sequence learning. The learning progress ,including six levels, 2 block , 4 block , 6 block , 8 block , 10 block , 12 block , was the independent variable. The dependent variable were conscious knowledge and unconscious knowledge, as well as the average response time and accuracy rate of each block. The result showed that only after BLK8 did the subject’s accuracy rate begin to be significantly higher than guess level(50%) via a one-sample t-test used for each level of the learning process, t（14）=2.71，p＜0.05，Cohen's d=0.694.What’s more , unconscious knowledge acquired in implicit sequence learning was more than conscious knowledge . Consciousness knowledge was increased gradually while unconscious knowledge was decreased in the whole learning process. This showed that implicit learning has time efficacy. Experiment 2 adopted one-factor completely random design to investigate the forgetting characteristics of knowledge acquired in implicit sequence learning. The retention interval , including nine levels,0h, 0.5h, 1h, 2h, 4h, 8h, 1day, 2days, 7days, was the independent variable. The independent variable was the same as experiment 1.The result showed that by means of taking the accuracy as the dependent variable and performing one-way analysis of variance ,the main effect of retention interval was significant, F（8，80）=4.45, p<0.001. Further one-sample t-test analysis found the judgment accuracy rate was significantly higher than guessing level(50%) while the retention interval was from 0 to 8 hours, respectively, t(12) = 2.84，p = .015；t（10）=2.76，p= .020；t(10) = 2.25, p=.049；t(8) = 2.91, p= .020；t（11）=3.68，p= .004；t（8）=2.77，p= .024；However, the accuracy rate decreased to guessing level at the interval of 24h, t(7)=1.60, p>0.05. Moreover, the accuracy rate was even lower than guessing level at the interval of either 48 hours or 7 days, t（7）= -2.59，p= .036,t（7）= -2.20，p>0.05. This experiment also showed that the main effect of retention interval of conscious knowledge acquired in implicit sequence learning reached a marginal significant, F（8，80）=1.81，p= .08. That conscious knowledge would be decreased after at the interval of 8h implied conscious knowledge could appear to be forgotten.Contrary to the above, the main effect of retention interval for unconscious knowledge acquired in implicit sequence learning was not significant, F（8，80）=1.30，p>0.05，it meant that unconscious knowledge was anti-forgetting.